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nf κ b oligonucleotide probe  (Promega)

 
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    Promega nf κ b oligonucleotide probe
    Nf κ B Oligonucleotide Probe, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nf+%CE%BA+b+oligonucleotide+probe/nf+%CE%BAb+oligonucleotide+probe/pmc04926984-106-14-19
    Average 90 stars, based on 1 article reviews
    nf κ b oligonucleotide probe - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Gel Shift:

    Article Title: Inhibitor of NF κ B Kinase Subunit 2 Blockade Hinders the Initiation but Aggravates the Progression of Crescentic GN
    Article Snippet: .. Nuclear miniature extracts were prepared, and gel shift assays were carried out using an NF κ B oligonucleotide probe (Promega, Heidelberg, Germany) end labeled with 32 P- γ -ATP (3000 Ci/mmol; GE Healthcare, Waukesha, WI). .. Afterward, 30 μ g nuclear protein was incubated for 30 minutes at room temperature with 100,000 cpm probe in 20 mM HEPES (pH 7.9), 0.3 mM EDTA, 0.2 mM EGTA, 80 mM NaCl, and 2 μ g poly(dI-dC)poly(Di-dC) (Amersham Pharmacia Biotech) in a total volume of 20 μ l. Where indicated, competition experiments were performed by adding unlabeled consensus oligonucleotides in a 100-fold molar excess to the binding reaction.

    Labeling:

    Article Title: Inhibitor of NF κ B Kinase Subunit 2 Blockade Hinders the Initiation but Aggravates the Progression of Crescentic GN
    Article Snippet: .. Nuclear miniature extracts were prepared, and gel shift assays were carried out using an NF κ B oligonucleotide probe (Promega, Heidelberg, Germany) end labeled with 32 P- γ -ATP (3000 Ci/mmol; GE Healthcare, Waukesha, WI). .. Afterward, 30 μ g nuclear protein was incubated for 30 minutes at room temperature with 100,000 cpm probe in 20 mM HEPES (pH 7.9), 0.3 mM EDTA, 0.2 mM EGTA, 80 mM NaCl, and 2 μ g poly(dI-dC)poly(Di-dC) (Amersham Pharmacia Biotech) in a total volume of 20 μ l. Where indicated, competition experiments were performed by adding unlabeled consensus oligonucleotides in a 100-fold molar excess to the binding reaction.



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